اثر اسانسهای فرار و ویتامین C بر روی تمایل به رسپتور مربوطه LDL مدلی برای جلوگیری از آترواسکلروز

          For several years , scientists tried to find antioxidant compounds that    inhibit the low density lipoprotein(LDL) oxidation reaction but have no    destructive effects. Vitamin C and vitamin E can inhibit LDL oxidation.    Volatile oils posses herbal sources and because of lipophilic properties can    enter LDL  and their antioxidant effects have not been known. The aim of    this study is possible use of herbal products such as volatile oils in preventing    or treating atherosclerosis. Because the probable effects of volatile oils on    the affinity of LDL to its receptor , this affinity can be influenced and the     modification of LDL and atherogenesis be prevented.           In this study , antioxidant properties of various concentrations of three    volatile oils include:P-cymol, Limonene and Thymol , and vitamin C  on the    affinity of  natural-LDL and oxidized-LDL(with Cu+2) to its receptor in bovine    adrenal tissue cells have been investigated in the presence of labeled-LDL    with fluoresein isothiocyanate (FITC). First, after purification of LDL-solvent of    normal serum and incubation it with these compounds, oxidation reaction    performed and LDL-solvent was labeled with FITC, and then all solvents were    added to the medium that contains adrenal tissue cells(with LDL-receptor).    Finally, fluorescence intensity of supernatant of each sample was    determined in spectrofluorometer. Also, the effects of the best    concentration of vitamin C (obtained in this study) with each one of these    compounds was studied.           Results showed that vitamin C in 100 micromolar concentration has the    greatest effect on the increasing affinity of LDL to its receptor (for natural-    LDL 18.5% and for oxidized-LDL 62%). Among the volatile oils used, “Thymol”    had the best effect (for natural-LDL 27% and for oxidized-LDL 50%). In    studying the effects of these three volatile oils with the best concentration of    vitamin C(100 micromolar) , “Thymol” had the highest effect on this affinity.           These finding showed that some compounds probably can change the    affinity of LDL to its receptor  and thus may prevent formation of    atherosclerosis .